Retroviral Recombination Is Nonrandom and Sequence Dependent
نویسندگان
چکیده
منابع مشابه
The recombination rate is not increased when retroviral RNA is missing an encapsidation sequence.
Retroviruses, as a result of the presence of two identical RNA molecules in their virions, recombine at a high rate. When nonhomologous RNA is present in the dimer RNA molecules, nonhomologous recombination occurs, although the rate is only 0.1% of the rate of homologous recombination. We developed a protocol to study transduction of cellular proto-oncogenes in a single cycle of retrovirus repl...
متن کاملRetroviral recombination during reverse transcription.
After mixed infection, up to half of related retroviruses are recombinants. During infection, retroviral RNA genomes are first converted to complementary DNA (cDNA) and then to double-stranded DNA. Thus recombination could occur during reverse transcription, by RNA template switching, or after reverse transcription, by breakage and reunion of DNA. It has not been possible to distinguish between...
متن کاملModification for the Mechanism of Retroviral Recombination
Figure 1: The original minus strand exchange model. (A) The retroviral virion contains two essentially identical RNA molecules. (B) An extended singlestranded DNA molecule is left tailing behind. This single-stranded DNA tail is free to form a hybrid duplex with another RNA molecule. (C) The original template will be displaced by branch migration of the second RNA. This second RNA molecule will...
متن کاملRetroviral recombination: review of genetic analyses.
Retroviruses package two copies of genomic RNA into one virion. One of the essential steps of replication is reverse transcription, in which the virally encoded enzyme reverse transcriptase (RT) uses the packaged RNA as a template to synthesize viral DNA. Because two copies of RNA are present in one virion, it is possible for RT to switch from one copy of the viral RNA to the other copy during ...
متن کاملRetroviral Vectors for Analysis of Viral Mutagenesis and Recombination
Retrovirus population diversity within infected hosts is commonly high due in part to elevated rates of replication, mutation, and recombination. This high genetic diversity often complicates the development of effective diagnostics, vaccines, and antiviral drugs. This review highlights the diverse vectors and approaches that have been used to examine mutation and recombination in retroviruses....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Virology
سال: 1998
ISSN: 0042-6822
DOI: 10.1006/viro.1998.9052